The BD Accuri ® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. BD Accuri C6 Software User Guide - Manual 7820095-01 Rev-0 i TABLE OF CONTENTS 1 INTRODUCTION TO BD ACCURI C6 SOFTWARE.....1 1.1 Starting BD Accuri C6 Software .....1 1.2 BD Accuri C6 Software Workspace .....2 1.3 Opening a New BD Accuri C6 Software Workspace .....2 1.4 Exiting BD Accuri C6 Software.....3 1.5 Using the Example BD Accuri C6 Software File.....3 2 VALIDATING THE … This portable and compact system holds the analytical power of a cytometer twice its size. BD CSampler SIP Collar Tool. The Accuri C6 is equipped with two excitation lasers: a blue solid state (488 nm) and a diode red (640 nm) providing up to 6 simultaneous detection pa-rameters, including 4 fluorescent colors plus FSC and SSC. The BD Accuri™ C6 personal flow cytometer brings immunology and stem cell studies to the benchtop. Flow cytometry (FCM) is a well-established technique (Marie et al. BD ACCURI FLOW CYTOMETER (B457) Rules and Guidelines. With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. This Cytometer is designed to deliver superior performance with ease of installation and operation for research applications.Cell counters for accurate and timely cell counts of dead cells, live cells and total cells are available at VWR. Read the interview » Noteworthy Get 40% off dendritic cell reagents. Cited in over 9,000 publications, the BD Accuri C6 Plus enables you to go beyond your current research potential. Learn more Class 1 Laser Product. BD Accuri™ C6 Flow Cytometer Quick Set Up Guide Please follow these directions for setting up your BD Accuri C6 Flow Cytometer. 1997; Gasol and del Giorgio 2000) used since the 1980s (Trask et al. Learn to adjust axes scaling factors in over 9. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O (0.22 µm filter), or equivalent, when the cytometer is not running samples. Histogram analysis of the forward scatter intensity (FSC) log channel values of the six polystyrene microsphere samples supplied in the Flow Cytometry Size Calibration Kit is shown. For more information on general flow cytometry, review the following: • Givan AL. As you probably know, FCS (Flow Cytometry Standard) files are created by flow cytometers. 21. Prior to measurement, the performance of the Accuri C6 was evaluated by analyzing eight peak rainbow particles (Spherotech, Lake Forest, IL). With two lasers, two scatter detectors, and four fluorescence detectors, it can handle most common assays with ease. When combined with the portfolio of BD reagents and best-in-class service and support, it is an ideal solution for life science researchers performing routine assays in cellular analysis, while also reducing operating costs. DI water Daily (if the cytometer is not shut down) Run the decontamination and cleaning cycles. BD Accuri cytometers use the C-Flow software, which produces FCS 3.0 compliant files. Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). the flow cytometer Accuri C6 (BD Biosciences, USA) in the clinical chemistry laboratory. All fluorescence signals of labeled spermatozoa were analyzed by the BD Accuri C6 Flow Cytometer and C6 Plus Flow Cytometer (Becton Dickinson, San Jose, CA, USA). These data files have a range of 16.7M, so the scale appears in 7 decades when log transformed. Even new flow cytometry users find it so easy to learn and use that We had a problem with its valves and it was solved in last November. • Melamed MR. Flow Cytometry and Sorting. At my lab, we have an Accuri C6 cytometer. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. CSampler. Manuals; Brands; BD Manuals; Laboratory Equipment; Accuri C6; BD Accuri C6 Manuals Manuals and User Guides for BD Accuri C6. BD Accuri C6 Instrument Manual (31 pages) Flow Cytometer. 3 VALIDATING THE PERFORMANCE OF THE BD ACCURI C6 FLOW CYTOMETER .....11 3.1 Running Validation Beads (Daily Start-Up) .....11 3.2 Saving Validation Bead Data .....15 3.3 Analyzing and Recording Validation Bead Data.....15 3.4 Monitoring Validation Bead Data.....19 3.5 Troubleshooting Validation .....20 4 MANUAL DATA ACQUISITION.....22 4.1 Viewing the Manual Collect Tab.....22 4.2 … If you can simplify cell analysis ITC Data Analysis in versions 8. With two lasers, two scatter detectors, and four fluorescence detectors, they can handle most common assays with ease. 1985) for enumeration and characterization of marine micro-organisms. Standard fluorochrome in use are identical to FACS Calibur. BD Accuri C6 Plus flow cytometer. Even more colors to choose from with selectable lasers and filter replacement. Preferences in FlowJo (v10+) are set by default to scale the data correctly according to the acquisition display and the format of the file. The cytometer will stop automatically when the time limit is reached. Flow Cytometer User Instructions. New York, NY: Wiley-Liss; 1990 (ISBN 0-471-56235-1). Interest Areas. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual . The reason for this overestimation was probably the different fluorescence patterns affected by the presence of alien particles, and thus a mathematical equation was proposed for correction of values for estimation of sperm concentration (21). For Research Use Only. Accuri C6 is a digital PC based bench top analyser, 2 laser system. Accuri C6 Flow Cytometer Instrument Manual Accuri C6 Software User’s Guide, Chapter 1, The Basics for a complete description of how to run and analyze bead results. Flow Cytometry: First Principles. 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